FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18456249
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27400.098.3046
ACGACGC29000.093.379695
CACGACG32600.083.506834
GACGCTC38750.069.693527
TACACGA44750.061.581152
ACGCTCT46400.057.8894548
CTACACG43400.056.890341
ACACGAC55900.049.2176863
CGCTCTT59100.045.3270769
CGGGTTA28551.8189894E-127.40512471
CGCTATA14202.265286E-46.9125051
TAACCCT743750.06.34745176
TCTTCCG98800.06.30199410-14
CGATGGA32454.6020432E-106.28245741
CGATGAG29201.254557E-86.2059591
CTAACCC797050.06.00474645
CTTCCGA112750.05.94608210-14
CGTTGAG26705.8522164E-75.93866061
ACCCTAA820900.05.8924082
CCTAACC816450.05.8883044
Produced by FastQC (version 0.11.5)