FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21184288
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG14300.090.233319
TGCGCGA33800.085.8596041
CGCCGAA31050.081.283161
TTTGCGC34050.080.102641
CGGTACG34350.076.226931
ACGACCG28500.072.73321
GTGCGAC34200.069.3304751
CGAACGC38250.068.454771
GCGCTAG34450.068.40511
GACGCGT35700.067.639841
ATAACGC41400.067.462681
TTACCCG41200.066.377871
GCGCGTA32550.065.6944961
GGCGACT43350.065.266931
CCGCTAT40550.065.1101151
GTTCGCG40950.064.0822148
GTCGGAC33200.062.8747751
TCACGCG30950.062.7707068
ACGCCGA40800.062.5719451
GTACCGT48550.062.4122668
Produced by FastQC (version 0.11.5)