FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17635551
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23350.094.270616
ACGACGC24650.090.179925
CACGACG27300.080.625014
GACGCTC35000.063.9229247
TACACGA40450.055.396722
ACGCTCT41900.053.567528
CTACACG41750.050.2616731
ACACGAC47900.046.403883
CGCTCTT53900.041.91029
CGGGTTA25701.05501385E-107.34269481
CGGATTA17105.9542763E-67.06272751
CGCTGAG35551.8189894E-126.5821941
CGCAAAT27001.6556442E-86.43002461
CGTAGAG25904.9365553E-86.4116751
CGGTTAG15355.875197E-46.39268881
CGTTGAG24851.5303522E-76.3788371
CGTGATG42700.06.36389831
TCTTCCG84950.06.20450110-14
TAACCCT718250.06.17980056
CTAACCC762500.06.03956465
Produced by FastQC (version 0.11.5)