FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18351691
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28400.087.329681
TTACGCG11100.084.898779
TTTGCGC29700.081.792951
CGCCGAA27200.076.743011
GTGCGAC32050.075.568721
GACGCGT28500.074.263261
CGGTACG27300.071.93281
ACGACCG30950.071.439561
GCGCGTA31500.067.883251
TCACGCG25150.067.158198
ATAACGC34150.066.662181
AACCGCG35550.066.2874451
GCGCTAG33500.066.2187351
CGCGGTA35550.065.264491
CCTTACG33600.064.939341
GTTCGCG38450.064.100828
TAGGCGC32750.063.7377051
GCCGGAT34150.063.680481
CGAACGC34700.063.5095441
TAAACCG39500.063.15721
Produced by FastQC (version 0.11.5)