FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20458072
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA31500.087.263971
TTACGCG14950.085.338759
ACGACCG29250.077.816581
TTTGCGC35450.077.540631
CGCCGAA30300.077.519971
CGGTACG34450.075.7807541
GTGCGAC36350.071.819731
GGCGACT43150.070.276321
GCGCTAG35000.070.018941
ACGCCGA41450.067.193611
GACGCGT31450.067.0549851
GTTACGC31850.066.458158
CGTTGCG16200.066.224937
CCGCTAT38100.066.0396651
TCCGCGA30850.064.85288
GCCGGAT41050.064.8368151
TCGCGAT22450.064.5785758
TAGGCGC39750.064.396061
TTACCCG37400.064.359121
CGAACGC34700.063.079861
Produced by FastQC (version 0.11.5)