FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18351691
Sequences flagged as poor quality0
Sequence length8
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1674491191.24451256290224No Hit
NAGTAGAG6072283.3088394960442606No Hit
TAGTAGAG1684700.9180080462339955No Hit
AAGGAGAG1309410.7135091801621987No Hit
GAGTAGAG1279950.6974561635764247No Hit
AAGTCGAG622680.3393038821327146No Hit
AAGTGGAG620430.33807783707779304No Hit
AGGTAGAG469040.25558407669353195No Hit
AAGAAGAG452690.24667481596110136No Hit
AAGCAGAG441320.24047920161689731No Hit
AAGTTGAG398380.2170808128798594No Hit
CAGTAGAG365600.19921869870193434No Hit
AAGTAGGG324100.17660497880004628No Hit
AAGTAGCG311100.16952116292716568No Hit
ACGTAGAG288260.15707544334742776RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGAA259930.14163817383368105No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)