FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18113539
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT53800.0117.065066
ACGACGC54950.0115.010785
CACGACG57150.0111.089774
GACGCTC65800.095.714227
TACACGA69850.091.101152
CTACACG67700.086.3326341
ACGCTCT74650.084.658258
ACACGAC79600.080.213563
CGCTCTT87050.072.84739
TCTTCCG116600.011.809587510-14
GTACACG24250.011.1957381
CTTCCGA126650.011.13569510-14
TTCCGAT152450.09.63145110-14
TCCGATC155250.09.22445115-19
CCGATCT164300.08.90161515-19
CGGATTA18756.8394E-108.4465621
TCGCCGG23200.08.435879145
CGATCTA40350.08.29819615-19
CGGGTTA24700.08.2438251
CGTAGAG30650.08.11980151
Produced by FastQC (version 0.11.5)