FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17672472
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12900.083.728239
TTTGCGC27550.078.393271
TGCGCGA27750.078.0903241
CGCCGAA27400.076.6992651
CGGTACG28450.075.146541
GTGCGAC30250.071.636581
GCGCTAG30450.067.106251
TAGGCGC33150.066.905261
GGCGACT36250.066.800571
GTTACGC29400.066.325468
GTTCGCG33750.065.0795068
ACGACCG26650.064.94171
CGTTGCG13800.064.084967
TCACGCG25850.063.9366268
GCCGGAT35950.063.919321
GCGCGTA32750.063.725691
GACGCGT31550.063.153171
TCGCGAT20600.062.9884838
ATAACGC33650.062.8856931
CGAACGC33950.062.7583851
Produced by FastQC (version 0.11.5)