FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17297048
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT28800.077.967046
ACGACGC30550.074.687435
CACGACG33450.067.995744
GACGCTC39050.057.5007677
TACACGA44100.051.6217462
ACGCTCT52200.043.436398
CTACACG47250.041.8972851
ACACGAC58950.039.19723
CGCTCTT64650.034.9597639
CGGATTA21451.6552804E-108.0101811
CGCTGAG41700.06.44923541
TAACCCT594200.06.27792936
CGGGTTA30355.5424607E-96.1535131
CTAACCC643350.06.0686165
ACCCTAA660550.05.9927832
CCCTAAC666950.05.9733453
CCTAACC660200.05.94664434
TCTTCCG95350.05.789171710-14
GTACACG27109.4124516E-75.78884361
CGTGATG53350.05.7410491
Produced by FastQC (version 0.11.5)