FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16623650
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10650.086.4347469
TGCGCGA24400.080.162831
ACGACCG24500.075.069331
TTTGCGC26750.074.7575151
GTGCGAC28100.074.542451
CGGTACG24950.073.715381
CGCCGAA24400.073.283181
GCGCTAG29250.069.366061
TTACCCG31650.067.795631
CCGCTAT31950.067.387481
GCGCGTA28500.065.8137051
TAGGCGC31500.065.8015061
GACGCGT26600.065.027151
CGAACGC30850.064.822161
TCACGCG23300.064.3948758
ATAACGC31150.063.729271
GCCGGAT33100.063.2822571
TCGACGG29400.063.0542641
GTTACGC26650.062.8277668
CCTTACG32950.061.576851
Produced by FastQC (version 0.11.5)