FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16623650
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26050.079.803056
ACGACGC28550.074.59175
CACGACG32450.065.403884
GACGCTC35850.058.5930677
TACACGA44000.048.607312
ACGCTCT43750.048.01948
CTACACG42450.042.2383231
ACACGAC55100.039.1755643
CGCTCTT56900.036.9218839
CGGGTTA29600.08.0766531
TAACCCT541150.06.70602856
CGTTGAG27805.506081E-96.4497011
CTAACCC584400.06.39571055
GACCCTA70200.06.1725421
ACCCTAA604700.06.13849972
CCCTAAC609450.06.12096263
AACCCTA594950.06.09950647
CCTAACC609450.06.025884
CGTAGAG31651.3938916E-85.90118741
TCTTCCG84350.05.89159910-14
Produced by FastQC (version 0.11.5)