FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14885284
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT20650.0101.424356
ACGACGC20850.0101.1391455
CACGACG22550.093.1931154
GACGCTC31000.067.326797
TACACGA34500.061.3435752
CTACACG31600.058.2663461
ACGCTCT35850.057.409268
ACACGAC38750.054.6131823
CGCTCTT49650.042.1824079
CGATGGA24300.08.6949441
CGCTAAG13701.9860036E-68.2620111
CGTAAAG20957.057679E-107.9241691
GTACACG20503.8598955E-97.73001771
CGGGTTA23557.494236E-97.0493141
TCTTCCG72350.06.810904510-14
CTTCCGA79250.06.67510310-14
GTTCCGA30554.1836756E-116.6416995
CGTAGAG23907.35854E-86.630351
CGGTGAG39451.8189894E-126.3122181
TAACCCT473200.06.1872996
Produced by FastQC (version 0.11.5)