FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19938256
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA29650.084.849251
TTTGCGC31450.081.380181
TTACGCG12300.078.384849
CGCCGAA30700.077.447251
GTGCGAC32950.073.041421
CGGTACG34000.070.999581
TCGTACG37450.068.717188
GCGCGTA34950.066.7812351
GCGCTAG37050.066.724811
ACGACCG29650.065.966611
CCTTACG37200.064.892091
TAGGCGC36750.064.697431
GGCGACT39450.063.402751
ATAACGC39150.063.14571
GTACGCT26400.062.8811348
CGCGGTA39350.062.4551961
GTTACGC32100.062.3293888
AACCGCG35950.062.294321
CGAACTA42750.061.3999061
TCGCGAT25900.061.296158
Produced by FastQC (version 0.11.5)