FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18640931
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC29600.082.290091
TGCGCGA28050.078.801621
TTACGCG11150.077.366299
ACGACCG31100.075.0481
CGCCGAA29450.072.83351
CGGTACG30400.071.7533341
GCGCTAG33150.069.749011
GACGCGT30650.067.609671
GTGCGAC32950.066.862351
AACGCGT25800.065.753153
TCGTACG37250.064.60988
GGCGACT40200.064.028261
CGAACGC34050.063.42111
GTACCGT47000.062.9287228
GCGCGTA32400.062.387021
TCTACGG46300.062.314448
GTTCGCG39100.062.109028
TTACCCG32250.061.775361
AACCGCG36100.061.6323431
TCGCGAT21800.061.5179528
Produced by FastQC (version 0.11.5)