FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18640931
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26900.095.874156
ACGACGC28100.093.070175
CACGACG29900.087.47084
GACGCTC38800.067.0290457
TACACGA42150.061.731682
ACGCTCT45900.056.186478
CTACACG41950.054.4574781
ACACGAC54400.047.6772543
CGCTCTT60250.043.88699
CGGGTTA29550.08.4198291
CGTTGAG27350.08.2701011
CGATGAG28250.07.7397411
CGCTTAG15751.7703424E-67.6592511
CGATGGG35750.07.5922991
GTTCCGA48250.07.20698365
CGTGATG49500.07.15878871
CGCCAAT16703.2942473E-56.77207331
CGCAAAG29202.728484E-106.713321
CGTAGAG30406.9485395E-106.44832041
CGATGGA34053.45608E-116.4213711
Produced by FastQC (version 0.11.5)