FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18085855
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT53250.0116.7641756
ACGACGC54250.0114.884415
CACGACG56850.0109.630244
GACGCTC65100.095.180397
TACACGA68050.091.6374362
CTACACG63650.087.066471
ACACGAC74600.083.740053
ACGCTCT74350.083.437258
CGCTCTT86650.071.677149
GTACACG26300.013.779671
TCTTCCG117150.011.5307710-14
CTTCCGA127000.011.00165510-14
TTCCGAT153500.09.35728710-14
CGATCTA43100.08.70992515-19
TCCGATC158300.08.67999215-19
CCGATCT171900.08.36426415-19
CGGGTTA26100.08.0997351
CGATGGA30100.07.77586131
CGGATTA17308.910356E-77.41918331
CGTGATG46300.06.84891221
Produced by FastQC (version 0.11.5)