FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18039155
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT50300.0112.820616
ACGACGC51850.0110.285725
CACGACG53500.0106.88354
GACGCTC61150.093.037537
TACACGA66750.086.2253342
CTACACG62900.084.855531
ACGCTCT69400.082.082718
ACACGAC75500.075.930623
CGCTCTT83050.069.028039
TCTTCCG114600.010.85251610-14
CTTCCGA127250.010.35461210-14
TTCCGAT150300.08.98844710-14
CGATCTA36650.08.46362115-19
TCCGATC150600.08.4313215-19
CCGATCT158000.08.375874515-19
CGAAGCG8450.0051243348.0294041
GTACACG24551.7524144E-86.7556751
CGGGTTA25152.8308932E-86.5945061
GCTCTTC202200.06.408909310-14
CGCTGAG36855.456968E-126.3419341
Produced by FastQC (version 0.11.5)