FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17705479
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10650.080.320299
TGCGCGA26800.076.7681961
GTGCGAC31100.076.4393541
TTTGCGC28200.075.534981
CGGTACG28850.073.833161
ACGACCG28400.072.69921
CGCCGAA26500.072.4248661
GCGCTAG32300.067.747561
GTTCGCG38450.064.856728
TTACCCG35800.064.779461
GTACCGT39100.063.7785388
CGAACGC29500.063.334561
ACGCCGA36600.063.1648831
ATAACGC31850.062.9983671
GCCGGAT34450.062.886391
TCGGCGC22050.062.7939228
GTTACGC24400.062.6881568
CCTTACG31400.062.512051
CCGCTAT34800.062.2539141
TCACGCG26100.061.3825078
Produced by FastQC (version 0.11.5)