FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19193852
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11150.082.5543069
TGCGCGA27750.077.592471
TTTGCGC31100.077.187041
CGGTACG28250.075.704151
CGCCGAA28600.075.540741
CCGCTAT38200.069.124831
TTACCCG33600.068.629491
ACGACCG29750.067.9749151
GCGCTAG32650.067.730021
GTACGCT29600.066.357268
GACGCGT28650.065.760631
GTGCGAC32100.065.038081
CGAACGC35000.064.845131
ATAACGC38700.064.472421
GGCGACT38600.063.5087281
TCACGCG29250.063.1866268
TAGGCGC35550.061.5910641
GCGCGTA29800.061.2700581
ACGCCGA37350.060.7651861
GTCGGAC27550.060.465091
Produced by FastQC (version 0.11.5)