FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16538315
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA24350.080.945111
TTACGCG10550.079.693189
CGCCGAA26200.073.8415151
GTGCGAC27900.073.5131761
TTTGCGC26750.072.595181
ACGACCG24000.072.428071
GCGCTAG29000.070.724751
CGGTACG25800.069.348281
TCGTACG33050.068.861078
CCGCTAT31950.067.153921
ATAACGC30800.066.11921
TCGACGG27850.066.071721
GGCGACT31550.065.4695361
GACGCGT21900.064.428481
GCGCGTA26650.063.588561
CGAACGC30300.063.369691
GCCGGAT32000.063.1851651
CGAACGT18800.062.85143
TAGGCGC31800.062.6676941
TTACCCG31800.062.6676941
Produced by FastQC (version 0.11.5)