FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16499499
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT20700.094.883646
ACGACGC21600.092.27235
CACGACG23500.085.119894
GACGCTC32350.061.161327
TACACGA35250.058.3917662
ACACGAC40050.051.030963
ACGCTCT38900.050.862958
CTACACG35800.049.2770771
CGCTCTT51750.038.512849
CGATGGA25400.09.4979161
GTACACG22052.0318112E-97.5218761
CGTTGAG22201.3766476E-76.7918671
CGTAGAG24501.6816557E-86.76968861
CGATGGG32859.640644E-116.42591241
CGGAAAG38201.8189894E-126.3153691
TCTTCCG81850.05.950212510-14
CGTTCCG19758.9477195E-55.87144952
CGACAAG20659.6374635E-55.84133431
TTACACG27359.419382E-75.7886071
TAACCCT439950.05.78224136
Produced by FastQC (version 0.11.5)