FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18697135
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11850.081.972699
TGCGCGA27450.078.117971
TTTGCGC31000.078.082531
CGCCGAA28500.075.239941
CGGTACG29450.072.319211
ACGACCG29300.071.697091
ATAACGC37400.069.968441
CGAACGC35200.068.765861
GCGCTAG30950.067.639931
GTGCGAC31550.067.505561
TAGGCGC35150.067.209291
GTTACGC27850.067.154828
GACGCGT29550.066.908721
CCGCTAT38450.066.356291
CGTTGCG13800.065.1366357
AACGCGT27250.063.8619883
CCTTACG35250.063.7192461
TCGACGG32200.063.65971
GTACGCT27200.063.1629148
TTACCCG36050.062.9101331
Produced by FastQC (version 0.11.5)