FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18513866
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28050.087.087721
TTTGCGC29700.079.067241
CGCCGAA28450.078.7080151
TTACGCG10950.078.118619
CGGTACG29650.073.3157041
GTGCGAC31900.072.4747851
ACGACCG27600.072.1758651
GCGCTAG31150.071.4190141
GGCGACT34650.067.981741
CCGCTAT34900.067.2864461
TTACCCG34450.066.899141
GCGCGTA31650.066.385711
GACGCGT30000.065.674771
AACCGCG36500.064.7352751
GTACCGT42500.062.2650688
GCCGGAT36100.062.2302861
TCGACGG33900.062.1940421
ACGCCGA36950.062.1760521
TAAACCG44250.062.105351
CGAACGC34500.061.7446061
Produced by FastQC (version 0.11.5)