FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18513866
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27800.096.458136
ACGACGC29250.091.6764455
CACGACG32000.084.250974
GACGCTC41000.065.9327247
TACACGA44100.062.6142272
CTACACG45450.054.8851471
ACGCTCT50950.053.0567558
ACACGAC54000.050.7317623
CGCTCTT60350.045.1526379
CGATGGA32550.010.8819711
CGCTGAG39250.08.2563771
CGTGATG48150.07.98242041
CGGTTAG16203.2252137E-77.90850831
CGGGTTA29050.07.52337271
CGCTAAT17701.0168827E-56.81251431
CGAAAAT36800.06.5533421
CGATGGG38050.06.53611951
AACGGGG36003.6379788E-126.48962931
CGCTGAT24401.11638656E-76.48619751
TCTTCCG101700.06.47056110-14
Produced by FastQC (version 0.11.5)