FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17533057
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24350.094.947676
ACGACGC25400.092.162975
CACGACG27700.084.771844
GACGCTC35400.065.719527
TACACGA38200.062.6213572
CTACACG40400.054.4565281
ACGCTCT44150.053.0227138
ACACGAC48250.048.967363
CGCTCTT55400.042.124649
CGGGTTA25559.640644E-117.37220761
CGTAGAG28702.5465852E-117.08810761
CGGTTAG15559.57544E-56.78337961
CGTTGAG24651.9219442E-86.72441051
CGATGGA30601.1823431E-106.6479971
TAACCCT665400.06.50255736
CTAACCC720850.06.2134275
TCTTCCG90050.06.116900410-14
ACCCTAA740500.06.0790882
CCTAACC738950.06.02198554
CCCTAAC748000.06.01695063
Produced by FastQC (version 0.11.5)