FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16935070
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC24850.080.466121
TGCGCGA24000.078.771451
GTGCGAC27450.076.5531
CGCCGAA26950.074.196021
CGGTACG25300.074.149111
ACGACCG26450.072.299751
GCGCTAG32000.071.575981
TTACGCG9200.068.5486159
TTACCCG32800.067.6133961
GACGCGT26100.067.418891
CCGCTAT33300.067.253241
ACGCCGA34850.066.1398541
GTTCGCG35000.065.446488
CGAACTA35600.065.154951
GCGCGTA26850.064.9940641
TAGGCGC29850.063.8210141
AACCGCG29550.062.9925541
TCGACGG29100.062.717311
GGCGACT33600.062.1082571
CGTTGCG11250.061.856597
Produced by FastQC (version 0.11.5)