FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16438297
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22000.096.195256
ACGACGC23500.092.21375
CACGACG24300.087.686054
GACGCTC32000.066.133637
TACACGA36600.059.4154852
CTACACG38200.053.85121
ACGCTCT41950.051.31118
ACACGAC42000.050.9052053
CGCTCTT53850.040.2413259
CGTAGAG26101.1732482E-96.9289411
CGCAAAT26751.9990694E-96.76057341
CGGGTTA22652.0007064E-76.65361831
GTTCCGA38150.06.64914235
CGCATAA15959.5346593E-46.1415611
GTACACG19955.891567E-56.04328631
TCTTCCG84000.06.039732510-14
TAACCCT399400.05.8612186
CTAACCC414150.05.84495165
CCCTAAC436250.05.73156363
CGAAAAT36851.6261765E-95.72554251
Produced by FastQC (version 0.11.5)