FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19850593
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGGAT1759109388.6174685058527No Hit
NGTCGGAT6671083.3606451958387336No Hit
GGTCGGAT6581943.3157397363393626No Hit
AGTCGGAT2117771.0668547785952793No Hit
CGTCGGAT1922120.9682934912826031No Hit
TGGCGGAT743550.3745731928512161No Hit
TGTCGGGT579690.2920265404665745No Hit
TGTCGGCT462570.2330257841667501No Hit
TGCCGGAT446540.22495045865884208No Hit
TGTCGGAG395750.1993643212573045No Hit
TGTCGGAA263500.1327416264088433No Hit
TGTCGGAC220240.11094882656654136Illumina NlaIII expression Adapter 1 (100% over 8bp)
TGTCGGTT206530.10404223188697688No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)