FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15410487
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA24150.087.636661
TTTGCGC24250.081.576881
CGCCGAA25200.077.924351
GCGCTAG26850.077.740541
ACGACCG23000.076.840181
GTGCGAC24400.073.921681
TTACGCG9550.072.870829
CGGTACG23400.071.486151
ATAACGC29450.066.4319461
GCCGGAT33100.066.357331
CCGCTAT30400.066.2698751
GCGCGTA25750.065.809511
GTTCGCG31250.064.488258
GACGCGT23950.064.378411
GGCGACT31400.063.46451
AACCGCG26000.062.9388921
ACGCCGA32500.062.882951
AACGCGT19900.062.6970833
TAGGCGC28600.062.557451
TTCCGCG13100.062.5308537
Produced by FastQC (version 0.11.5)