FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15410487
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21900.097.9585656
ACGACGC22800.095.362365
CACGACG25050.087.085634
GACGCTC32050.066.935157
TACACGA35500.063.095682
CTACACG37350.055.8930171
ACGCTCT40550.053.082748
ACACGAC41850.052.646063
CGCTCTT49250.043.8528489
GTACACG20002.0412699E-87.53647761
CGATGAG24301.9190338E-97.1332911
TCTTCCG75700.06.644539410-14
CTTCCGA84450.06.31654310-14
CGAAAAG29903.274181E-96.30140261
CGGATTA15607.280535E-46.28039741
CGCTGAG30856.544724E-96.1073561
CGCAAAG25151.3127828E-65.9932221
CGGTAAT17755.294528E-45.9442641
GTTCCGA31502.4418114E-85.7520155
CGATGGA24503.3232845E-55.53700351
Produced by FastQC (version 0.11.5)