FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17867514
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10450.081.852489
TGCGCGA27150.081.148011
TTTGCGC27700.079.799261
ACGACCG30850.078.486351
GTGCGAC30300.076.7913741
CGGTACG30350.073.07121
CGCCGAA29250.071.841771
GCGCTAG30350.070.675421
GACGCGT29300.068.989381
CGAACGC33850.068.3083041
GGCGACT34750.068.003871
TTACCCG32600.067.804891
ATAACGC33700.067.102011
AACGCGT25900.064.6767963
TCGCGAT20650.064.589798
CGCGGTA36500.063.5481451
ACGCCGA37000.063.0824241
GTACCGT41200.062.6352588
CTTACCG39400.061.2698671
GCGCAAC38950.061.2310141
Produced by FastQC (version 0.11.5)