FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18150252
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10500.091.1186459
TGCGCGA26000.078.057391
GTGCGAC30400.076.570151
ACGACCG27150.075.8227841
TTTGCGC29350.074.600461
CGGTACG29750.074.575471
CGCCGAA28950.074.3748861
GGCGACT35650.069.578971
GCGCTAG31600.067.216971
ATAACGC33400.067.0791241
TTACCCG34800.066.679851
CGAACGC33100.065.9289861
TCGCGAT20800.065.85998
GACGCGT27100.065.762751
GTTCGCG35650.065.669798
ACGCCGA37000.064.681091
GTACCGT40000.063.7830548
TCGTACG33650.063.7572068
GCGCGTA28400.063.5208781
TAGGCGC33900.062.4419441
Produced by FastQC (version 0.11.5)