FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18150252
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT53800.0115.072046
ACGACGC55250.0112.7075
CACGACG55750.0111.428134
GACGCTC63000.098.032867
TACACGA69850.089.393382
CTACACG63950.086.3323441
ACGCTCT72450.085.144158
ACACGAC75750.082.5800553
CGCTCTT85150.072.359999
TCTTCCG116150.011.55655410-14
CTTCCGA122500.011.37121310-14
GTACACG24750.011.2907611
TTCCGAT148250.09.5816910-14
TCCGATC150900.09.01006415-19
TTACACG34450.08.7693511
CCGATCT159700.08.76744615-19
CGATCTA38750.08.55687815-19
CGTTAGG21156.9503585E-97.4990391
CGATGGA32750.07.14902931
GCGTCGG33750.07.079903145
Produced by FastQC (version 0.11.5)