FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15491315
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24100.095.842316
ACGACGC24650.094.290715
CACGACG25700.090.4307864
GACGCTC32600.070.849327
TACACGA37800.061.7758062
ACGCTCT39200.059.473958
CTACACG36200.057.7950251
ACACGAC41050.057.1438063
CGCTCTT51050.045.668549
CGGGTTA22705.456968E-128.3182781
CGTAGAG24403.092282E-117.73872571
TCTTCCG78650.06.903923510-14
CGGATTA16151.519504E-46.54748961
CTTCCGA90000.06.35503610-14
CGCTGAT20257.069838E-55.96779971
CGTAAAG22801.0117641E-55.9628921
CGCTGAG31801.2163582E-85.93789051
TAACCCT538600.05.90176346
CGAAAAG29701.14483555E-75.84912161
CTAACCC570400.05.71233655
Produced by FastQC (version 0.11.5)