FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17348650
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT19850.085.7219856
ACGACGC22100.076.994635
CACGACG24100.070.299734
GACGCTC30450.055.6406447
TACACGA37150.046.8480222
ACGCTCT38700.044.339778
CTACACG39900.040.6795351
ACACGAC49100.034.9473423
CGCTCTT49100.034.653139
CGTGATG47600.08.7230051
CGGGTTA26353.6379788E-127.73559241
CGTAGAG29751.8189894E-127.35904461
CGCTGAG35300.07.27134231
CGATGGA31153.6379788E-127.02830121
CGTTGAG23859.567884E-96.9637741
CGATGAG26751.9190338E-96.7732621
TAACCCT594550.06.4790026
CTAACCC641400.06.3331355
CGCCAAA26356.9123416E-86.30307531
CGAGGAT23252.1338783E-66.16937351
Produced by FastQC (version 0.11.5)