FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18640931
Sequences flagged as poor quality0
Sequence length8
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1603322686.01086501527205No Hit
NAGTAGAG7195873.860252473441375No Hit
TAGTAGAG5858063.142579091140888No Hit
CAGTAGAG4394852.357634390685744No Hit
GAGTAGAG3906392.095598122218252No Hit
AAGGAGAG1120510.6011019513993158No Hit
AAGTGGAG454290.24370563895118758No Hit
AAGTCGAG368850.19787101835203402No Hit
AGGTAGAG350050.18778568516776334No Hit
AAGAAGAG339850.18231385546140372No Hit
AAGCAGAG338160.1814072483826049No Hit
AAGTAGGG255030.13681183627577398No Hit
AAGTAGCG244930.1313936519586924No Hit
AAGTTGAG236890.12708056266073833No Hit
ACGTAGAG198060.10625005800407716RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGAA188060.1008855190762736No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)