FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17262288
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT20200.086.098726
ACGACGC22100.080.340745
CACGACG24500.073.062434
GACGCTC30850.056.143857
TACACGA35550.050.7895852
ACGCTCT37550.046.319718
CTACACG39400.039.8583071
ACACGAC47950.037.935723
CGCTCTT51550.034.1621069
CGATGGA29950.09.8314931
CGTAGAG28250.08.5523061
CGTGATG45700.08.4256941
CGGGTTA26050.08.11525251
CGATGAG28850.07.58933831
GTACACG22954.4165063E-97.2375521
TCGCCGG25005.455331E-86.379281145
CGTAAAG21754.7390004E-66.2483441
CGATGGG35308.367351E-116.20261761
TAACCCT650350.05.91676336
CGTCAAC16600.0015042585.9127151
Produced by FastQC (version 0.11.5)