FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16623701
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT19500.089.568556
ACGACGC21000.084.887135
CACGACG23650.075.3754654
GACGCTC29200.060.309187
TACACGA34600.051.9460562
ACGCTCT35850.049.526578
CTACACG35400.044.536951
ACACGAC42500.042.6302223
CGCTCTT49650.036.1986589
CGGGTTA22550.011.0393831
CGCAAAG30301.8189894E-127.2199271
CGTAAAT26401.4679244E-96.85779861
CGATGGA30306.366463E-106.47303771
CGCTGAG34354.3655746E-116.3686691
TAACCCT569900.06.2184666
CGTAGAG26709.094765E-86.21568251
CGGATTA16009.769636E-46.1291581
CGATGGT39455.456968E-126.11899951
CGGTTAG16100.00105303086.0910891
CTAACCC612650.05.9968285
Produced by FastQC (version 0.11.5)