FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17396220
Sequences flagged as poor quality0
Sequence length8
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1331096376.5164098867455No Hit
TAGTAGAG11470606.5937312818531835No Hit
CAGTAGAG9669935.558638600799484No Hit
GAGTAGAG7350304.22522823923818No Hit
NAGTAGAG7235694.159346110821776No Hit
AAGGAGAG1048540.6027401355007007No Hit
AAGTGGAG448730.2579468413252994No Hit
AAGAAGAG401860.23100420666098725No Hit
AAGTCGAG389010.22361754450104676No Hit
AAGCAGAG342440.19684736109338696No Hit
AAGTAGGG313560.18024605345299152No Hit
AGGTAGAG305580.17565885002604015No Hit
AAGTAGCG282930.16263878014879093No Hit
AAGTTGAG259230.14901513087325868No Hit
AAGTAGAA229970.13219538497443697No Hit
ACGTAGAG203080.1167380040031685RNA PCR Primer, Index 17 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)