FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18189567
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28300.079.648581
TTACGCG11950.077.647479
CGCCGAA27850.076.497151
ACGACCG27750.075.986741
TTTGCGC31350.074.9148251
CGGTACG28650.073.092141
GTGCGAC30400.070.797991
GCGCTAG30100.070.054221
ATAACGC36750.065.2919161
GACGCGT26850.064.722671
CCTTACG33450.064.12521
TAGGCGC34300.063.172051
TAAACCG43300.063.1397321
CCGCTAT33400.063.1326981
CGAACGC31700.062.619011
TTACCCG37900.062.543361
GGCGACT36500.062.352561
ACGCCGA36400.062.32411
GCCGGAT35800.061.3375931
CCGATAA38650.061.3297121
Produced by FastQC (version 0.11.5)