FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17988920
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26400.085.089721
TTACGCG13700.083.074449
CGCCGAA29050.077.327671
ACGACCG26550.074.751631
TTTGCGC26850.073.916421
GTGCGAC29950.071.120271
CGGTACG27900.070.8740541
ATAACGC33450.067.8080141
TTACCCG35050.066.994181
GCGCTAG33450.066.721351
CGAACGC31350.066.5528951
GTTACGC28400.066.1104058
AACGCGT26050.064.85493
CCGCTAT37200.063.9039041
TTCCGCG17800.063.5320637
TAAACCG41600.063.08651
GGCGACT35550.062.98451
GTTACCG26900.062.7902228
GTACCGT38100.062.0270278
GCCGGAT36900.061.8622741
Produced by FastQC (version 0.11.5)