FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17988920
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC28050.096.311455
CGACGCT28500.094.536886
CACGACG31000.087.613614
GACGCTC40500.066.16817
TACACGA42350.065.213242
ACACGAC50300.054.8588753
ACGCTCT49500.054.7231148
CTACACG45350.051.369321
CGCTCTT61850.044.0306369
CGGGTTA28500.09.5540621
CGTGATG47100.08.0293341
GTACACG24152.3646862E-117.8298471
CGTTGAG27851.8189894E-127.60436961
CGATAGG14353.1626445E-57.37915331
CGTTAGG20201.816934E-77.11430741
CGGATTA16051.809082E-57.0688151
TCTTCCG99900.06.612076310-14
TAACCCT655950.06.55871736
GTTCCGA49400.06.30439575
CGATGGA31251.2223609E-96.29294161
Produced by FastQC (version 0.11.5)