FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18362234
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10600.086.130939
CGCCGAA27650.079.151151
TGCGCGA30900.077.649981
GTGCGAC33950.075.8139951
TTTGCGC28050.073.875071
TTACCCG35900.070.4807741
ATAACGC33750.069.800271
CGGTACG31000.069.190461
ACGACCG28250.067.68981
GGCGACT35950.066.135521
GCCGGAT33750.065.4916151
GACGCGT30550.065.449661
GTTACGC28250.065.4057858
GCGCTAG31000.065.203211
CGAACGC34400.064.8882141
TCGCGAT21350.064.483738
GCGCGTA31050.063.9273871
TCGTACG36900.063.2301068
AACCGCG32300.063.0291441
TAGGCGC33550.062.847991
Produced by FastQC (version 0.11.5)