FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18362234
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26300.094.232916
ACGACGC27700.089.737965
CACGACG30550.081.603994
GACGCTC39400.063.27337
TACACGA41700.060.5113562
ACGCTCT43900.056.7875828
CTACACG43650.052.3918271
ACACGAC51250.048.503063
CGCTCTT59450.041.8125279
CGCTGAG38700.08.5811771
CGATGGA31400.08.172491
CGATGGG38000.07.54753541
GTTCCGA50050.07.52941235
CGTAGAG33600.07.18812851
GTACACG23456.853952E-97.0808431
CGTTAGG20753.0123374E-76.91099551
CGGGTTA28451.4551915E-106.89757161
CGCCAAT14502.9404738E-46.76675561
CGTAAAG26158.572897E-96.6383671
CGCTGAT24058.254756E-86.59036351
Produced by FastQC (version 0.11.5)