FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19850593
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11900.081.018439
TGCGCGA31000.078.59591
CGCCGAA31400.077.131431
ACGACCG29700.076.648681
TTTGCGC32650.076.628811
CGGTACG31200.074.12921
GCGCTAG32650.071.728131
GTGCGAC33100.067.017571
ATAACGC39150.066.507111
TTACCCG38900.065.812721
TCGTACG39350.065.029318
GACGCGT31850.064.8523561
GGCGACT36900.064.2551651
CCTTACG38200.064.16281
TCGACGG31950.063.283541
CCGCTAT39750.062.758681
CTTACCG44950.062.456031
TCTACGG47950.062.43688
GCGCGTA36800.062.0581251
CTAACGA41250.061.8870771
Produced by FastQC (version 0.11.5)