FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19153982
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC31300.080.8247151
TTACGCG10800.077.189529
GTGCGAC31300.074.786091
TGCGCGA28000.074.513191
CGGTACG30700.072.932591
CGCCGAA30650.072.57721
ACGACCG28450.070.5238651
GCGCTAG29150.068.830321
TCACGCG26700.067.611058
TTACCCG37100.066.621481
TTCCGCG19050.066.600117
TCGTACG36300.065.9078148
GACGCGT32100.064.3165361
TCCGCGA27850.064.038268
TAGGCGC37150.063.7922741
CCTTACG34250.063.6751481
ATAACGC39900.063.2216571
TCGCGAT24250.062.483338
AACCGCG38050.062.0923351
CGAACTA40500.061.926051
Produced by FastQC (version 0.11.5)