FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18425867
Sequences flagged as poor quality0
Sequence length8
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1560450984.68805836924798No Hit
NAGTAGAG7817274.242552060101161No Hit
TAGTAGAG6353403.4480874088584272No Hit
CAGTAGAG5170982.806369979768116No Hit
GAGTAGAG4350962.3613325766434765No Hit
AAGGAGAG1017650.55229422854295No Hit
AAGTGGAG467750.2538550831827886No Hit
AAGTCGAG369610.20059300330345378No Hit
AAGAAGAG320910.17416276802605815No Hit
AAGCAGAG314240.17054285695213148No Hit
AGGTAGAG300580.1631293659071782No Hit
AAGTAGGG266300.14452508530534816No Hit
AAGTTGAG238480.129426745563723No Hit
AAGTAGCG232100.12596422192779314No Hit
ACGTAGAG190710.10350123551852404RNA PCR Primer, Index 17 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)