FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19054404
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10900.080.470069
CGCCGAA30600.078.1900941
CGGTACG30350.078.115311
TTTGCGC32250.076.219171
TGCGCGA28950.073.351931
ACGACCG27650.072.0663761
GTGCGAC33200.068.123911
TTACCCG37150.067.144831
GGCGACT37050.066.73721
TTCCGCG19600.066.57247
TAGGCGC34650.066.112651
ATAACGC36000.065.6535341
GACGCGT28700.065.37551
GCGCTAG31700.064.2356341
CCTTACG36250.063.3951951
TCCGCGA28550.063.2220738
CCGCTAT38050.062.689731
CGAACGC36700.062.6178671
TCACGCG27950.062.504428
TCGTACG37000.062.3018468
Produced by FastQC (version 0.11.5)