FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17396220
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT28450.078.416686
ACGACGC30700.075.031545
CACGACG34550.065.412834
GACGCTC39500.056.8457877
TACACGA46050.050.0644952
ACGCTCT48500.045.8561068
CTACACG43650.043.7964131
ACACGAC61800.037.273063
CGCTCTT62750.035.442579
CGGGTTA32450.08.2845821
CGATGGA35250.08.0502111
GTACACG30700.08.0270951
CGGATTA22653.2259777E-86.923631
TAACCCT680400.06.4087336
CGTAGAG33904.0017767E-116.38823841
CTAACCC741950.06.14088735
GACCCTA74100.06.0466641
AACCCTA737450.05.98161367
CCTAACC771450.05.94363744
ACCCTAA761200.05.94317052
Produced by FastQC (version 0.11.5)