FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17380316
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21150.084.605956
ACGACGC21550.083.372925
CACGACG23600.076.1327444
GACGCTC30800.058.0967457
TACACGA35000.051.978052
ACGCTCT39550.044.693848
CTACACG38550.041.6702651
ACACGAC50050.036.3330153
CGCTCTT52900.033.962659
CGTAAAG23350.010.0125771
CGATGGA32500.09.978291
CGTGATG49000.08.0034691
CGTTAGG22052.0172592E-97.52462961
CGCCAAT14202.8595241E-57.43550971
CGATGGG36050.07.1128671
CGTAGAG28902.1282176E-106.78494831
CGCTAAG15801.1645237E-46.6825461
CGGATTA16301.7472252E-46.477561
GTACACG21704.6476925E-66.2558141
CGGTAGT19554.3333454E-56.17226081
Produced by FastQC (version 0.11.5)